Phase separation during blood spreading

Abstract Blood pools can spread on several types of substrates depending the surrounding environment and conditions. Understanding influence these parameters spreading blood provide crime scene investigators with useful information. The focus present study is phase separation, that is, when serum spreads outside main pool. For this purpose, constant initial masses wooden floors were either varnished or not created at ambient temperatures $$21~^{\circ }\hbox {C}$$ 21 ? C , $$29~^{\circ 29 $$37~^{\circ 37 a relative humidity varying from 20 to 90%. range covers almost all worldwide indoor cases. same whole donor was used for experiments. As result, an increase in found result final pool area. In addition, three different experimental temperatures, levels above 50%. This separation more significant substrates, does lead any changes drying morphology. phenomenon explained by competition between coagulation evaporation.